Postmortem human brain specimens were obtained from the Brain Collection of Y.L.H. (table S1) (45, 46). Brain samples used in the study were from clinically unremarkable adult male Caucasian subjects, not diagnosed with any neurological or psychiatric condition at the time of death and with negative toxicology for common drugs of abuse or for therapeutic agents. Specimens had been collected at autopsy within 24 hours after death by the personnel of the Department of Forensic Medicine (Semmelweis University, Hungary) under approved local ethical guidelines. The cause of death was determined by a forensic pathologist. All subjects used in our study died of non–suicide- and non–drug abuse–related causes, such as accident or cardiac failure. Immediately after autopsy, brains were cut coronally in slabs and kept frozen at −70°C. There were no significant differences in brain pH among specimens. Postmortem intervals for all subjects were <24 hours. Tissue samples were dissected from dorsolateral PFC, Brodmann area 9.

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