The full-length hTRPV6 (residues 1 to 725) and rTRPV6 (residues 1 to 727) were introduced into a plasmid Eric Gouaux (pEG) BacMam vector (27), with a C-terminal thrombin cleavage site (LVPRG), followed by a streptavidin affinity tag (WSHPQFEK). Human CaM complementary DNA was cloned into the bacterial expression vector pCDFDuet-1 with a C-terminal His tag.

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