Cell viability assays were performed on cells under three conditions: without any treatment (control), flowing through the device (10 μl/min) without SAWs, and flowing through the device with SAWs (with an excitation of 15 Vpp at 12 MHz). For each condition, live/dead cell staining was conducted using calcein AM for live cells and SYTOX orange for dead cells (Molecular Probes, Life Technologies). The cell viability was obtained by counting live and dead cells. Cells were counted as dead if they showed any amount of SYTOX orange stain.

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