Polystyrene beads (10 μm; Bangs Laboratories Inc.) were suspended in deionized water to an approximate concentration of 1 × 106 ml−1. U937 cells [purchased from the American Type Culture Collection (ATCC)] were cultured in RPMI 1640 medium (Gibco, Life Technologies) containing 10% fetal bovine serum (Gibco, Life Technologies) and 1% penicillin-streptomycin (Mediatech). HeLa cells (purchased from ATCC) were cultured in DMEM (Gibco, Life Technologies) containing 10% fetal bovine serum (Gibco, Life Technologies) and 1% penicillin-streptomycin (Mediatech). Both the U937 and HeLa cells were maintained in a cell culture incubator (Nu-4750, NuAire) with a temperature of 37°C and a CO2 level of 5%. Before each experiment, U937 (or HeLa) cells were harvested and resuspended in fresh RPMI 1640 medium (or DMEM) to an approximate concentration of 1 × 106 ml−1.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.