Samples of 0.5 ml were mixed with 0.5 ml of fixation medium (3% paraformaldehyde, 2% glutaraldehyde, and 400 mM NaCl, final) and stored at 4°C. Samples were adhered to silicon chips coated with poly-l-lysine (0.01%; Sigma-Aldrich). After three washes in 0.1 M cacodylate buffer, samples were postfixed with 1% OsO4 for 1 hour, followed by three washes in 0.1 M cacodylate buffer and three washes in Milli-Q water. Samples were dehydrated by a series of increasing concentration of ethanol (30 to 100%). Ethanol was replaced by liquid CO2 and critical point dried in BAL-TEC Critical Point Dryer 030. Last, samples were coated with gold/palladium (Edwards, S150) and imaged using the high-tension mode of XL30 ESEM.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.