Flow cytometry analyses were performed using an Eclipse iCyt flow cytometer (Sony Biotechnology Inc., Champaign, IL, USA) equipped with 405- and 488-nm solid-state air-cooled lasers and with a standard optic filter setup. E. huxleyi cells were identified by plotting the chlorophyll fluorescence (663 to 737 nm) against side scatter and were quantified by counting the high-chlorophyll events. For bacterial counts, samples were fixed with a final concentration of 0.5% glutaraldehyde for at least 30 min at 4°C, then plunged into liquid nitrogen, and stored at −80°C until analysis. After thawing, samples were stained with SYBR Gold (Invitrogen) that was diluted 1:10,000 in tris-EDTA buffer, incubated for 20 min at 80°C, and cooled to room temperature. Samples were analyzed by flow cytometry (excitation, 488 nm; emission, 500 to 550 nm). For algal cell death analysis, samples were stained with a final concentration of 1 μM SYTOX Green (Invitrogen), incubated in the dark for 30 min at room temperature, and analyzed by flow cytometry (excitation, 488 nm; emission, 500 to 550 nm). An unstained sample was used as control to eliminate the background signal.

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