Sulfitobacter D7 and Marinobacter D6 were isolated from a coculture of E. huxleyi 379 with CAM at 7 days of growth. Bacterial populations in cocultures were stained with the live nucleic acid fluorescent marker SYTO 13 (Molecular Probes). Two distinct subpopulations were observed in CAM-treated cultures (fig. S1B) and were sorted at room temperature on the basis of green fluorescence intensity (530/30 nm) in purity mode using a BD FACSAria II cell sorter equipped with a 488-nm laser. Sorted populations were independently plated on marine agar 2216 plates (Difco) and incubated in the dark at 18°C. Sulfitobacter D7 and Marinobacter D6 were each isolated from a single colony and streaked three times from a single colony to ensure isolation of a single bacterial strain. For identification, DNA was extracted from a single colony of Sulfitobacter D7 and Marinobacter D6 using REDExtract-N-Amp Plant PCR kit (Sigma-Aldrich) according to the manufacturer’s instructions and was used as a template for PCR with general primers for bacterial 16S ribosomal RNA (rRNA): 5′-agtttgatcctggctcag-3′ (forward) and 5′-taccttgttacgacttcacccca-3′ (reverse) (58). Amplicons were paired-end sequenced using the ABI 3730 DNA Analyzer and manually assembled. Sulfitobacter D7 and Marinobacter D6 were grown in marine broth 2216 (Difco) and stored in 15% glycerol at −80°C.

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