High-resolution micro-CT scanning was conducted using an Xradia Micro-XCT scanner at the Imaging and Analysis Centre, Natural History Museum, London. S. canicula embryos were stained with 0.1% phosphotungstic acid in 70% EtOH for 3 days to enhance contrast. Scans were rendered using the three-dimensional volume exploration tool Drishti (https://github.com/nci/drishti). SEM was undertaken using a Hitachi TM3030Plus Benchtop SEM scanner at 15,000 V.

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