Coimmunoprecipitation was performed as previously described (5). Briefly, cells were lysed in 50 mM tris, 150 mM NaCl, and 1% CHAPS buffer containing protease and phosphatase inhibitors. Lysates were precleared with protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology) for 3 hours, followed by overnight incubation at 4°C with the respective Abs. Agarose A/G beads were then added for 4 hours, pelleted, and washed as described (5). Each experiment was repeated three times.

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