Molecular model generation and visualization was achieved through Schrodinger Maestro 10.4, performed according to the standard protocol. BCL9, β-cat, and TCF4 (3SL9) protein structures were imported from PDB (Protein Data Bank), and the β-cat molecule was optimized for glide docking with Protein Preparation Wizard (Prep Wizard). hsBCL9CT-24 was constructed in Maestro and converted to a three-dimensional structure through LigPrep, retaining stereochemical properties. Subsequently, glide and receptor grid generation were applied at the β-cat N′ helices 2 and 3 regions, followed by glide and ligand docking of hsBCL9CT-24 at its binding site for visualization.

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