1,1′,2-Tris-norsqualenic acid (100 mg, 0.25 mmol) and TEA (34.79 mg, 0.3 mmol) were dissolved in 1.5 ml of anhydrous THF under argon, and ethyl chloroformate (27 mg, 0.25 mmol) was added to the mixture at 0°C. The reaction was allowed to warm at room temperature and kept under stirring for 1 hour. A solution of LENK (138 mg, 0.25 mmol) in 1.5 ml of anhydrous DMF was then added to the reaction, and the mixture was kept under stirring for 2 days. The solvents were removed in vacuo, and the crude product was purified twice using silica gel chromatography [purification with gradient eluent DCM/EtOH (100:0 to 90:10) and then simple filtration with EtOH/AcOEt (40:60) to remove the ammonium salt]. The pure bioconjugate was obtained with 73% yield.

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