Whole-cell patch-clamp recordings were carried out as described previously on an upright microscope (BX51WI) with an EPC 10 amplifier (2, 40). Worms were immobilized and dissected on a Sylgard-coated coverglass to expose muscles. Bath solution contained 145 mM NaCl, 2.5 mM KCl, 5 mM CaCl2, 1 mM MgCl2, 20 mM glucose, and 10 mM Hepes (320 mOsm; pH adjusted to 7.3). Pipette solution contained 115 mM KCl, 15 mM KOH, 1 mM MgCl2, 10 mM Hepes, 0.25 mM CaCl2, 20 mM sucrose, 5 mM EGTA, 5 mM Na2ATP, and 0.5 mM NaGTP (315 mOsm; pH adjusted to 7.2). Series resistance and membrane capacitance were both compensated during recording. Voltage was clamped at −60 mV.

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