Life-span experiments were performed at 20°C, as previously described (27, 39). Worms were age synchronized by picking L4 hermaphrodites, and the next day was scored as day 1. Each worm was scored for viability every day, and the worms that crawled off the plate, exploded, or bagged were censored at the time of the event. The life-span data were processed using the Prism 7 and SPSS Statistics software. P values were calculated with the log-rank (Kaplan-Meier) method and Cox proportional hazard regression, as indicated. RNAi experiments were done using OP50(xu363), an RNAi-compatible OP50 bacterial strain, as described above and previously (38). The detailed life-span data were described in table S1.

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