First-generation adenovirus serotype 5 expressing Escherichia coli β-galactosidase under the control of a cytomegalovirus promoter was amplified in human embryonic kidney 293 cells (ATCC CRL-1573) and purified from secondary lysates according to established methods (19). Preparations with a ratio of infectious to physical viral particles of 1:100 were used in the studies summarized here.

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