Dulbecco’s PBS, Trizma base [2-amino-2-(hydroxymethyl)-1,3-propanediol], potassium ferricyanide, potassium ferrocyanide, glutaraldehyde (grade I, 25% in water), FBS (qualified, U.S. origin), glycerol, and d-sorbitol [USP (United States Pharmacopeia) grade] were purchased from Sigma-Aldrich (St. Louis, MO). Poly(maleic anhydride-alt-1-octadecene) substituted with 3-(dimethylamino)propylamine was purchased from Anatrace (Maumee, OH). DMEM was purchased from Mediatech (Manassas, VA). Hydroxypropyl methylcellulose 4*KM was provided by the Dow Chemical Company (Midland, MI). Penicillin (10,000 IU) and streptomycin (10,000 μg/ml) were purchased from Gibco Life Technologies (Grand Island, NY). 5-Bromo-4-chloro-3-indolyl-β-d-galactoside (X-gal) was purchased from Gold Biotechnology (St. Louis, MO). All other chemicals were of analytical reagent grade and purchased from Thermo Fisher Scientific (Pittsburgh, PA) unless specified otherwise.

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