Approximately 500 μg of venom was separated on a Phenomenex Gemini NX-C18 column (250 × 4.6 mm; particle size, 3 μm; pore size, 110 Å) using a gradient of 15 to 45% solvent B (90% ACN and 0.05% TFA) over 30 min at a flow rate of 1 ml min−1. Fractions were collected on the basis of absorbance at 214 nm. MALDI-MS was used to confirm the identity of the peptide/s present in each fraction, and purity was assessed by rerunning an aliquot of each under the same conditions. Purified fractions containing MIITX1-Mg1a, MIITX1-Mg2a, and MIITX2-Mg1a were dried by vacuum concentration and resuspended in 80 μl of pure water from which 7 μl-aliquots were used for calcium imaging experiments.

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