To obtain the soluble form of FL BCR as a full-size antibody, VH and VL were cloned into the pFUSE antibody expression vectors (InvivoGen) and produced using the FreeStyle 293 Expression System (Thermo Fisher Scientific). All measurements were made using a Biacore T200 apparatus (GE Healthcare Life Sciences). Biotinylated peptides were immobilized on SA chips following the manufacturer’s recommendations. The flow rate of HBS-EP buffer during all measurements was maintained at 25 μl/min. A recombinant malignant BCR sample was serially diluted with HBS-EP buffer within the range of 0.0039 to 2 μM and tested at a standard association/dissociation time of 300/300 s. The dissociation constants were calculated using the Biacore T200 1.0 evaluation software.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.