To obtain the soluble form of FL BCR as a full-size antibody, VH and VL were cloned into the pFUSE antibody expression vectors (InvivoGen) and produced using the FreeStyle 293 Expression System (Thermo Fisher Scientific). All measurements were made using a Biacore T200 apparatus (GE Healthcare Life Sciences). Biotinylated peptides were immobilized on SA chips following the manufacturer’s recommendations. The flow rate of HBS-EP buffer during all measurements was maintained at 25 μl/min. A recombinant malignant BCR sample was serially diluted with HBS-EP buffer within the range of 0.0039 to 2 μM and tested at a standard association/dissociation time of 300/300 s. The dissociation constants were calculated using the Biacore T200 1.0 evaluation software.

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