Cell lines were cultured in media supplemented with 10% fetal bovine serum (Gibco), 10 mM Hepes, penicillin (100 U/ml), streptomycin (100 μg/ml), and 2 mM GlutaMAX (Gibco). The 293T lentiviral packaging cell line (Clontech) and HEp-2 cell line were cultured in Dulbecco’s modified Eagle’s medium (Gibco). Human HEp-2 (CCL-23), Jurkat (TIB-152), and Raji (CCL-86) cell lines were obtained from the Institute of Cytology RAS culture collection (St. Petersburg, Russia). The Jurkat, Jurkat-FL, Raji, and Raji-FL cell lines were cultured in RPMI 1640 (Gibco). Human peripheral blood mononuclear cells (PBMCs) were isolated from the blood of healthy donors by gradient density centrifugation on a Ficoll-Paque (GE Healthcare), washed, and then resuspended in serum-free RPMI 1640.

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