Oligonucleotides used in this work (table S1) were synthesized and high-performance liquid chromatography–purified by Sangon Biotechnology Co., Ltd. T4 DNA ligase, Phi29 DNA polymerase, nicking endonuclease Nb.BbvCI, and deoxynucleotide triphosphates (dNTPs) were obtained from New England BioLabs Inc. FastFire qPCR PreMix (SYBR Green) was obtained from Tiangen Biotech Co., Ltd. (China). Hemin, ThT, dimethysulfoxide (DMSO), hydrogen peroxide (H2O2), 2,2′-azino-bis(3-ethylbenzothiozoline-6-sulfonicacid) diammonium salt (ABTS2−), and bovine serum albumin (BSA) were purchased from Sigma-Aldrich. Ultrapure water obtained from a Millipore filtration system was used throughout. All other chemical reagents used in this work were of analytical grade without further purification. The buffer solutions used were as follows: TE buffer [10 mM tris-HCl, 0.1 mM EDTA, and 0.1 M NaCl (pH 7.8)], KT buffer [100 mM MES, 50 mM tris-HCl, 40 mM KCl, 0.05% Triton X-100, and 1% DMSO (pH 6.2)], HBS-EP buffer [10 mM Hepes, 15 mM NaCl, 3 mM EDTA, 0.005% Tween 20, and 0.1% BSA (pH 7.4)], 5× TBE buffer [446 mM tris, 446 mM boric acid, and 10 mM EDTA (pH 8.3)], and 5× d-PAGE loading buffer [1 M NaOH (1 ml), formamide (95 ml), and bromophenol blue (0.05 g)]. The oligonucleotide stock solutions (10 μM) were prepared with 10 mM TE buffer and kept frozen. A stock solution of hemin (100 nM) was prepared with DMSO and stored at −20°C in the dark. All other chemical reagents used in this work were of analytical grade without further purification.

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