As mentioned above, we first assembled the simple triangle DNA origami monomers named triangle A and triangle C, respectively. Each kind of simple triangle DNA origami monomer was prepared according to Liu et al. (20). A molar ratio of 1:10 between the long M13 scaffold and each required staple strand (“extended staples,” selected “anchoring staples,” and the rest staple strands) was used, and DNA origami was assembled in 1× TAE-Mg2+ buffer [40 mM tris, 20 mM acetic acid, 2 mM EDTA, and 12.5 mM magnesium acetate (pH 8.0)] by the reported annealing program (95°C for 3 min, 95° to 15°C, 0.1°C/10 s) (21). The triangle DNA origami monomers were subsequently purified four times with Microcon centrifugal filtration devices (100-kDa molecular weight cutoff filters, Millipore) to remove the excess staple strands. The concentration of each purified DNA origami monomer was estimated from the optical absorbance at 260 nm. Then, the two kinds of purified DNA origami triangular monomers were mixed in a 1:1 ratio and annealed from 45° to 15°C at a speed of 0.1°C/min. The trapezoid-shaped super-origami was prepared in the same way.

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