HIE monolayers were treated with 5 μM ruxolitinib or dimethyl sulfoxide (DMSO) control for 1 hour at 37°C and then infected with NR-labeled EV71 or treated with IFN-λ3 in the presence of ruxolitinib or DMSO. Similarly, HIE monolayers were treated with 80 μM dynasore or DMSO for 30 min at 37°C before infecting with NR-labeled EV71 in the presence of dynasore or DMSO.

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