HIE monolayers were treated with 100 to 500 ng of recombinant IFN-β, IFN-λ1, or IFN-λ3 (1598-IL-025, 5259-IL-025, and 8499-IF-010; R&D Systems) added to both the apical and basolateral compartments for ~20 hours before initiating infections, as described above.

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