Total RNA was extracted as described above. RNA quality was assessed by NanoDrop and an Agilent bioanalyzer, and 1 μg was used for library preparation using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) as per the manufacturer’s instructions. Sequencing was performed on an Illumina NextSeq 500. RNA-seq FASTQ data were processed and mapped to the human reference genome (hg38) using CLC Genomics Workbench 11 (Qiagen). Differential gene expression was performed using the DESeq2 package in R (18). Files from HIE used in the current study were deposited in sequence read archives.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.