Total RNA was extracted as described above. RNA quality was assessed by NanoDrop and an Agilent bioanalyzer, and 1 μg was used for library preparation using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) as per the manufacturer’s instructions. Sequencing was performed on an Illumina NextSeq 500. RNA-seq FASTQ data were processed and mapped to the human reference genome (hg38) using CLC Genomics Workbench 11 (Qiagen). Differential gene expression was performed using the DESeq2 package in R (18). Files from HIE used in the current study were deposited in sequence read archives.

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