NPCs were differentiated from hiPSCs using the PSC Neural Induction Medium (Thermo Fisher Scientific) with modifications. In brief, hiPSCs were replated as clumps in Geltrex (Thermo Fisher Scientific)–coated six-well plates (Thermo Fisher Scientific) in mTeSR1 (StemCell) on day 0. From day 2, the medium was switched to PSC Neural Induction Medium and changed daily. On day 11, NPCs were harvested using Neural Rosette Selection Reagent (StemCell). NPCs were maintained in Neural Expansion Medium (Thermo Fisher Scientific) and were passaged every 4 to 6 days in the presence of 5 μM ROCK inhibitor (R&D Systems) until the fourth passage (P4). P4 NPCs were characterized by positive IF staining for Nestin and Pax6 (Fig. 3, C and D).

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