The hiPSC lines used for deriving neurons were generated using the Sendai virus method at the Rutgers University Cell and DNA Repository–NIMH Stem Cell Center from cryopreserved lymphocytes of the Molecular Genetics of Schizophrenia (MGS) collection (58). The patient line was derived from a 29-year-old male patient with SCZ (cell line ID: 07C65853), and the other two lines were derived from unaffected controls (cell line IDs: 05C39664 and 05C43758, designated as CD07 and CD09, respectively). All three lines do not have large CNVs associated with SCZ. The NorthShore University HealthSystem Institutional Review Board approved the study. hiPSCs were cultured using the feeder-free method in Geltrex (Thermo Fisher Scientific)–coated plates in mTeSR1 medium (StemCell). The media were changed daily, and cells were passaged as clumps every 5 days using ReLeSR (StemCell) in the presence of 5 μm ROCK inhibitor (R&D Systems). hiPSCs were characterized by positive IF staining of pluripotent stem cell markers OCT4, TRA-1-60, NANOG, and SSEA4 (Fig. 3, A and B).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.