The hiPSC lines used for deriving neurons were generated using the Sendai virus method at the Rutgers University Cell and DNA Repository–NIMH Stem Cell Center from cryopreserved lymphocytes of the Molecular Genetics of Schizophrenia (MGS) collection (58). The patient line was derived from a 29-year-old male patient with SCZ (cell line ID: 07C65853), and the other two lines were derived from unaffected controls (cell line IDs: 05C39664 and 05C43758, designated as CD07 and CD09, respectively). All three lines do not have large CNVs associated with SCZ. The NorthShore University HealthSystem Institutional Review Board approved the study. hiPSCs were cultured using the feeder-free method in Geltrex (Thermo Fisher Scientific)–coated plates in mTeSR1 medium (StemCell). The media were changed daily, and cells were passaged as clumps every 5 days using ReLeSR (StemCell) in the presence of 5 μm ROCK inhibitor (R&D Systems). hiPSCs were characterized by positive IF staining of pluripotent stem cell markers OCT4, TRA-1-60, NANOG, and SSEA4 (Fig. 3, A and B).

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