The silicon foils that have captured the cell were incubated with 10 μl of the CuPc-Abs-MGT nanoprobes at 37°C for 60 min. To minimize the background, they were washed with PBS buffer (pH 7.4) to remove the free nanoprobe in the solution. Then, after the recognition approach and washing process, the silicon foils were subjected to Raman spectroscopy to collect the sample spectra (each sample was collected 10 times at different points). For the Raman mapping mode, a 50 μm by 50 μm area was selected for imaging. We collected 32 pixels by 32 pixels in 2 hours.

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