Chitosan precipitate formation
This protocol is extracted from research article:
Conjoined-network rendered stiff and tough hydrogels from biogenic molecules
Sci Adv, Feb 1, 2019; DOI: 10.1126/sciadv.aau3442

Ten milliliters of 2 wt % chitosan solution was mixed with equal-volume phosphates, which have different phosphate group numbers (the molar concentrations of phosphate group were identical to those in 20 wt % sodium phytate, pH 7.4). The precipitate phase was allowed to settle down for 24 hours. The upper polymer-depleted equilibrium phase was removed from the dense precipitate phase by centrifugation. The precipitate phase was lyophilized and weighed. The content of chitosan in precipitate phase was obtained by thermogravimetric analysis (TA Instruments Pyris 1; from 25° to 650°C at a rate of 10°C/min under N2 environment). The chitosan coacervate ratio was calculated using Eq. 1Embedded Image(1)where mchitosan precipitate is the weight of chitosan in the precipitate phase and mtotal chitosan is the weight of chitosan in the original chitosan solution.

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