Aggregation reactions in the presence and absence of clusterin and proSP-C Brichos were followed by recording ThT fluorescence emission at 480 nm after excitation at 440 nm. Samples (100 μl) were incubated in the Corning (Corning, USA) 96-Well (COSTAR) Half-Area Black with Clear Flat Bottom Polystyrene NBS Microplate, and measurements were recorded in a FLUOstar OPTIMA plate reader (BMG Labtech, Ortenberg, Germany) at 21° or 37°C under quiescent conditions (10). For seeded reactions, fibrils were freshly prepared before each experiment and mixed with freshly purified monomeric Aβ(M1-42) at the desired concentrations. The concentrations of clusterin, monomeric, and fibrillar Aβ(M1-42) for the different experiments are reported in the main text and Supplementary Materials as well as in the captions of the figures. The concentration of ThT was 20 μM in all experiments that we carried out at 37°C, unless otherwise stated.

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