Mice were anesthetized with 5% chloral hydrate and then fixed on a stereotaxic apparatus (RWD Life Science). Stainless steel guide cannulas (RWD Life Science) were bilaterally implanted into the target brain areas, and the tips of cannulas were at following coordinates (in millimeters): BLA: AP −1.40, ML ±3.40, DV −3.50; mPFC: AP +1.70, ML ±1.65, DV −1.70, angled at 30°; dHPC: AP −1.90, ML ±1.90, DV +0.17, angled at 30°; vHPC: AP −3.16, ML ±3.20, DV −3.75. The cannulas were fixed to the skull using acrylic cement and two skull screws. Stainless steel obturators (33 gauges) were inserted into guide cannulas to avoid obstruction until drug infusion. Animals were allowed to recover from surgery for 2 weeks before behavioral tests. Mice were handled and habituated to the infusion procedure several days before drug injection. During drug infusion, mice were briefly head restrained, while the stainless steel obturators were removed and injection cannulas (33 gauges, RWD Life Science) were inserted into guide cannulas. Injection cannulas protrude 1.00 mm from the tips of guide cannulas. Infusion cannula was connected via PE20 tubing to a microsyringe driven by a microinfusion pump (KDS 310, KD Scientific). Drugs were infused bilaterally into the target brain areas at a flow rate of 0.15 μl per min. After finishing drug injection, the injection cannulas were left in place for 2 min to allow the solution to diffuse from the cannula tip. The stainless steel obturators were subsequently reinserted into guide cannulas, and the mice returned to their home cage for 30 min before behavioral tests.

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