The PMC3A-coated substrate was prepared using a spin-coating method. PMC3A was dissolved in methanol, and the polymer solution was prepared at 0.2% (w/v). The polymer solution was spin-coated twice onto PET substrates (ϕ = 14 mm, thickness = 125 μm) using Mikasa Spin Coater MS-A100 at the consecutive rates of 500 rpm for 5 s, 2000 rpm for 10 s, a ramp up for 5 s, 4000 rpm for 5 s, and a ramp down for 4 s and was then dried. The human platelet adhesion tests were performed according to a previously reported procedure (31). Platelet-rich plasma (PRP) and platelet-poor plasma (PPP) were obtained from human whole blood using a two-step centrifugation, and then a plasma solution containing platelets (4 × 107 cells/cm2) was prepared by mixing the PRP and PPP solutions. The plasma solution (200 μl) was placed on each polymer substrate, and the substrate was incubated for 1 hour at 37°C. After 1 hour, each polymer substrate was rinsed with PBS, and then the platelets that had adhered on the polymer substrates were fixed by immersion in 1% glutaraldehyde in PBS for 120 min at 37°C. Finally, each polymer substrate was rinsed with PBS and Milli-Q water. The platelet adhesion number was evaluated using SEM.

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