Neurons at DIV10 were transfected with PCAGGS-IRES-eGFP. At DIV16, neurons were first treated with 0.1% DMSO, GW9508 (20 μM), or GW1100 (20 μM) for 30 min and then washed, fixed, stained, and mounted. To label the dendrites and spines, fixed neurons were incubated with rabbit anti–green fluorescent protein (GFP) primary antibody (Invitrogen) and subsequently with fluorophore-conjugated goat anti-rabbit Alexa Fluor 488 secondary antibody. Alexa Fluor 488 images were captured on a confocal laser scanning microscope (Leica, Wetzlar, Germany). The number of spines was counted in a blinded manner, and dendrite length was measured in Image-Pro Plus 6.0.

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