Preparation of SUVs
This protocol is extracted from research article:
Nano-bio-computing lipid nanotablet
Sci Adv, Feb 22, 2019; DOI: 10.1126/sciadv.aau2124

The lipid solution in chloroform was mixed in a round-bottom flask to have 97.2 mole percent (mol %) dioleoylphosphatidylcholine, 0.3 mol % biotinylated dioleoylphosphatidylethanolamine (DOPE), and 2.5 mol % poly(ethylene glycol) 1000 (PEG 1000)–DOPE. Chloroform was removed using a rotary evaporator, and the lipid film formed inside the flask was thoroughly dried for 15 min under a stream of N2. The dried mixture was resuspended in deionized (DI) water to have a total concentration of 2 mg/ml. The lipid solution went through three freeze-thaw cycles between −78° and 40°C. To generate uniform SUVs, the solution was extruded more than 11 times through a polycarbonate membrane with a pore diameter of 100 nm at 30°C. The resulting SUV solution was kept at 4°C until use.

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