The microbiome of both the sputum samples and the media from the WinCF columns after incubation in the pH and oxygen experiments were analyzed using 16S rRNA gene amplicon sequencing. For the sputum samples, an aliquot of the sample was frozen at −80°C before sequencing in batch with all other samples. DNA extraction, 16S rRNA gene variable region 4 (V4) polymerase chain reaction, and amplicon preparation for sequencing were performed according to protocols benchmarked for the Earth Microbiome Project found here http://www.earthmicrobiome.org/protocols-and-standards/ (see Supplementary Methods).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.