TG, Dulbecco’s modified Eagle’s medium:Nutrient Mixture F-12 (DMEM/F12), high-glucose DMEM with glucose (4500 mg/liter), Fura-2 AM, Cy3- and Cy5-conjugated secondary antibodies, and penicillin-streptomycin were from Invitrogen. Fetal bovine serum (FBS), rabbit polyclonal anti-Orai1 antibody, mouse anti-actin antibody, rabbit anti-CCT2 antibody, ATP, and protease inhibitor cocktails were from Sigma-Aldrich. Mouse monoclonal anti-CCT5 antibody (GT639) was from GeneTex. Rat monoclonal anti-CCT1 antibody (91A, sc-53454) was from Santa Cruz Biotechnology Inc. Purified monoclonal anti-HA.11 Epitope Tag antibody (#MMS-101P) was from Covance. Rabbit anti-Orai1 antibody (ab78471) was from Abcam. Mouse anti-GM130 was from BD Transduction Laboratories. Rabbit anti-EEA1 antibody and rabbit anti–Na,K-ATPase antibody (#3010) were from Cell Signaling. Rabbit anti-IP3R antibody was from Affinity BioReagents. Horseradish peroxidase–conjugated goat anti-rabbit IgG and goat anti-mouse IgG antibodies were from Jackson ImmunoResearch Laboratories. Enhanced chemiluminescence detection reagents and immobilized streptavidin gel were from Pierce. IRDye 800CW goat anti-mouse IgG and IRDye 680RD goat anti-rabbit IgG antibodies were obtained from LI-COR Biosciences.

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