Rats were randomized to three groups (n = 6 rats per group): (i) direct exposure (no gel, MPT given dermally, 100 mg/kg per day for 4 days), (ii) sham gel (220 mg of sham gel applied daily dermally 30 min before the MPT exposure), and (iii) poly-Oxime gel [220 mg of gel applied daily dermally before applying MPT (100 mg/kg per day) daily for 4 days] (Fig. 4A). In all animals before exposure, 200 μl of blood was collected using retro-orbital puncture and evaluated for AChE activity as an internal control. Immediately after mortality or on day 30, animals were sacrificed, and their organs were collected to quantify the AChE level as described above. Similarly, another set of a single application of gel experiments was performed (n = 6 rats per group). In this experiment, 220 mg of sham gel or poly-Oxime gel was applied only once, i.e., on day 0, and on the same gels, these animals received MPT (100 mg/kg per day) for 4 days (Fig. 4D).

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