A solution of poly-Oxime (2 mg/ml) in 1% acetic acid was used to study its catalytic ability to cleave pesticides. The reactions were studied spectrophotometrically by monitoring the hydrolysis of pesticides (MPT, methyl paraoxon, or chlorpyrifos) at 25 ± 0.1°C and pH 8.2 by measuring the absorbance at 400 nm as a function of time. Pseudofirst-order rate constants were obtained by using an excess of nucleophile (2.5 × 10−4 M) over the substrate (2.5 × 10−5 M). For all the kinetic runs, the absorbance/time result fits very well to the first-order rate equation. To study temperature stability, the same reactions were carried out at 20°, 30°, and 40°C.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.