Pyridine-2-aldoxime, bromoacetic acid, chitosan (medium molecular weight), dimethyl formamide, potassium carbonate, O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HBTU), parathion methyl (PESTANAL), methyl paraoxon (PESTANAL), chlorpyrifos (PESTANAL), carbopol 940, glycerin, propylene glycol, triethanolamine (TEA; S. D. Fine-Chem Limited, Bangalore), snake skin dialysis membrane (MWCO, 3.5 kDa; Thermo Fisher Scientific), trifluoroacetic acid, pNP (Alfa Aesar), isoflurane (Isotroy), Triton X-100, EDTA (Thermo Fisher Scientific), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), acetylthiocholine iodide (ASChI), and deionized water were used. Unless mentioned otherwise, all chemicals were procured from Sigma-Aldrich.

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