After conducting a 24-hour time course, as described in the “Measuring time courses” section, cultures that had been exposed to cefotaxime doses of 0, 2, and 200 μg/ml were diluted 10-fold in fresh M9 (antibiotic free) and incubated at 37°C for 3 hours (fig. S11). The recovered cultures were then used to run another time course using the same antibiotic concentrations used in the previous round of treatment.

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