Overnight cultures of ESBL isolates I to IV and sensitive cells (MG1655) were prepared by inoculating a colony in LB for 12 hours at 37°C. ESBL cultures were washed and diluted, as in the “Measuring time courses” section, and the appropriate amount of cefotaxime (Sigma) was added to achieve 50 μg/ml in M9. Exogenous Bla (Sigma) was prepared in phosphate-buffered saline (PBS) and added to the ESBL cultures to achieve concentrations ranging from 1 to 100 μg/ml. MG1655 was washed and diluted, as in the “Measuring time courses” section, and the appropriate amount of carbenicillin (Genesee) was added to achieve 100 μg/ml. Exogenous Bla was prepared in PBS and added to the MG1655 cultures to achieve concentrations ranging from 1 to 20 μg/ml. Time courses were measured by a Tecan Spark and a Tecan M200 plate reader for the ESBL isolates and MG1655, respectively, as in the “Measuring time courses” section.

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