One milliliter of overnight culture was washed (centrifuged for 5 min at 13,000 rpm, discarded the supernatant, and resuspended in 1 ml of fresh M9), and the OD was adjusted to 0.5 OD600 by adding the appropriate volume of M9 (fig. S9). The culture was then diluted 1000-fold in fresh M9, and the appropriate amount of cefotaxime (Sigma) was added to achieve a range of concentrations from 0 to 300 μg/ml. A 96-well plate (Costar) was loaded with 200 μl of culture per well and topped with 50 μl of mineral oil (Sigma) to prevent evaporation. The plate was loaded into a Tecan Infinite M200 PRO microplate reader (chamber temperature maintained at 30°C), and OD600 readings were measured every 10 min for 48 hours with intermittent plate shaking. Unless otherwise noted, each condition tested consisted of four technical replicates that, when averaged, did not need to include error bars.

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