We characterized bacterial isolates from a library assembled by the Duke Hospital’s Division of Infectious Diseases. This library consists of approximately 80 isolates that have been identified as ESBL producers. Unless otherwise noted, Klebsiella pneumoniae isolate DICON 005 was used. As a sensitive bacteria control, Escherichia coli MC4100 cells were used. Unless otherwise indicated, experiments were conducted in M9 medium [1× M9 salts (48 mM Na2HPO4, 22 mM KH2PO4, 862 mM NaCl, and 19 mM NH4Cl), 0.4% glucose, 0.2% casamino acids (Teknova), 0.5% thiamine (Sigma), 2 mM MgSO4, and 0.1 mM CaCl2]. For overnight cultures, we inoculated single colonies from an agar plate into 2 ml of M9 and incubated them for 12 hours at 30°C.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.