All strains and plasmids used in this study are summarized in Table 1. All strains were routinely maintained in lysogeny broth (LB) at 30°C. Antibiotics were supplemented into the growth media whenever necessary [ampicillin (100 μg/ml), kanamycin (50 μg/ml), and gentamicin (80 μg/ml); Sigma-Aldrich]. Stock cultures were maintained in LB medium with 20% glycerol at −80°C. Seed culture was prepared by transferring 0.5 ml of stock culture to 50 ml of LB medium in a 200-ml Erlenmeyer flask and incubated for 12 hours at 30°C with shaking at 200 rpm.

Amp, ampicillin; Km, kanamycin; Gm, gentamicin.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.