All strains and plasmids used in this study are summarized in Table 1. All strains were routinely maintained in lysogeny broth (LB) at 30°C. Antibiotics were supplemented into the growth media whenever necessary [ampicillin (100 μg/ml), kanamycin (50 μg/ml), and gentamicin (80 μg/ml); Sigma-Aldrich]. Stock cultures were maintained in LB medium with 20% glycerol at −80°C. Seed culture was prepared by transferring 0.5 ml of stock culture to 50 ml of LB medium in a 200-ml Erlenmeyer flask and incubated for 12 hours at 30°C with shaking at 200 rpm.

Amp, ampicillin; Km, kanamycin; Gm, gentamicin.

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