Immunohistochemistry was performed as described previously. Antibodies against the following proteins were used at the indicated dilutions: Chx10 (sheep/1:1000, Exalpha Biologicals), microphthalmia-associated transcription factor (Mitf) (mouse/1:1000, Exalpha Biologicals), and pMLC2 (rabbit/1:50, Cell Signaling Technology). DAPI (4′,6-diamidino-2-phenylindole) was used for counterstaining the nuclei (Molecular Probes). Stained sections were analyzed with an LSM780 Confocal Microscope (Carl Zeiss).

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