Immunohistochemistry was performed as described previously. Antibodies against the following proteins were used at the indicated dilutions: Chx10 (sheep/1:1000, Exalpha Biologicals), microphthalmia-associated transcription factor (Mitf) (mouse/1:1000, Exalpha Biologicals), and pMLC2 (rabbit/1:50, Cell Signaling Technology). DAPI (4′,6-diamidino-2-phenylindole) was used for counterstaining the nuclei (Molecular Probes). Stained sections were analyzed with an LSM780 Confocal Microscope (Carl Zeiss).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.