Quantification of epithelial curvature, height, and signal intensity
This protocol is extracted from research article:
Strain-triggered mechanical feedback in self-organizing optic-cup morphogenesis
Sci Adv, Nov 21, 2018; DOI: 10.1126/sciadv.aau1354

The epithelial curvature, height, and signal intensity were measured from the immunostaining images of sliced tissues and/or the confocal images of whole tissues, where the curvature is measured by a sequential three-point method (12).

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