Signal-to-background ratio. SBR is defined here as SBR = μsigbg, where μsig and μbg are the average values of the signal and the background, respectively. In the case of cells and fluorescent micropatterns, two small regions of interest were defined—one containing fluorescent structures and the other corresponding to the background. In the images of beads, the highest-intensity pixels in the beads were used as signal value, and the maximum intensity pixels in the rest of the image were used as background noise. Uncertainty is given by the SD of all averaged measurements. All values of SBR presented in this work are summarized in table S1.

Cell size. As the cells used here appear approximately spherical, their diameter was estimated by taking a measurement of their size in two orthogonal directions and averaging the obtained values. Their diameter was determined by fitting a Gaussian function to the intensity values and measuring its full width at half maximum. Table S2 shows the diameters of the cells that appear in the different images presented in this work. Deviations in cell size between reconstructed and reference images are expressed as a percentage error.

Bead spacing. The central pixel for each individual bead was located, and the distance between beads was measured in the reference image and in all retrieved images with different CRs (table S3). Deviations in bead spacing between reconstructed and reference images are expressed as a percentage error.

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