The voltage-gated K+ channel blocker was injected locally with a programmable automatic pump into the pyramidal layer of CA1 hippocampus to induce local epileptic seizure. One injection of 250 nl of 25 or 50 mM 4AP dissolved in ACSF was injected to the left hemisphere of the mouse hippocampus. Neuronal activity was recorded 1 hour before 4AP injection for control. In every experiment, neuronal activity was recorded for a minimum of 2 hours following drug administration.

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