Where indicated, data were subjected to a two-tailed Student’s t test to resolve statistical significance. The measured TFI values of GFP molecules and single-labeled mRNA transcripts were plotted into histograms and fitted with a Gaussian function according to the formula Embedded Image to determine the mean value xc of the intensity distribution in OriginPro 8. The mean TFI value for single GFP molecules was related to the mean single transcript intensities via conversion of the exposure time at identical imaging settings. The comparison of DNA ligase IV knockdown and control live cell experiment datasets was performed using Fisher’s exact test for 2 × 2 contingency tables and a χ2 test for contingency tables, both at a level of significance of P = 0.05.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.