Whole-cell protein extracts were prepared by cell lysis with SDS–polyacrylamide gel electrophoresis (PAGE) buffer [80 mM tris-HCl (pH 6.8), 16% glycerol, 4.5% SDS, 450 mM dithiothreitol, and 0.01% bromophenol blue], with benzonase (200 U/ml; Sigma-Aldrich) and 50 μM MgCl2 and boiling for 5 min. Equal amounts of protein extracts were resolved by SDS-PAGE and transferred to a nitrocellulose membrane. Immunoblotting was performed with the following antibodies: rabbit anti-DNA ligase IV (ab193353, Abcam), mouse anti–α-tubulin (T5168, Sigma-Aldrich), rabbit anti–total histone H3 (ab1791, Abcam) and using the secondary antibodies goat anti-mouse horseradish peroxidase (HRP) (170-6516, Bio-Rrad) and goat anti-rabbit HRP (170-6515, Bio-Rad).

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