Gel electrophoresis of DNA origami objects
This protocol is extracted from research article:
Sequence-programmable covalent bonding of designed DNA assemblies
Sci Adv, Aug 17, 2018; DOI: 10.1126/sciadv.aau1157

Samples were electrophoresed on 2.0% agarose gels containing 0.5× tris-borate- EDTA and 5 mM MgCl2 for around 2 hours at 90-V bias voltage in a gel box immersed in a water or ice bath, unless otherwise stated. Samples were loaded on the gel at a monomer concentration of approximately 5 nM. The electrophoresed agarose gels were scanned using a Typhoon FLA 9500 laser scanner (GE Healthcare) at a resolution of 25 μm/pixel. The resulting 16-bit tif images were analyzed using ImageJ 1.440.

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