Gel electrophoresis of DNA origami objects
This protocol is extracted from research article:
Sequence-programmable covalent bonding of designed DNA assemblies
Sci Adv, Aug 17, 2018; DOI: 10.1126/sciadv.aau1157

Samples were electrophoresed on 2.0% agarose gels containing 0.5× tris-borate- EDTA and 5 mM MgCl2 for around 2 hours at 90-V bias voltage in a gel box immersed in a water or ice bath, unless otherwise stated. Samples were loaded on the gel at a monomer concentration of approximately 5 nM. The electrophoresed agarose gels were scanned using a Typhoon FLA 9500 laser scanner (GE Healthcare) at a resolution of 25 μm/pixel. The resulting 16-bit tif images were analyzed using ImageJ 1.440.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.