Folding of DNA origami objects
This protocol is extracted from research article:
Sequence-programmable covalent bonding of designed DNA assemblies
Sci Adv, Aug 17, 2018; DOI: 10.1126/sciadv.aau1157

The reaction mixtures contained scaffold DNA at a concentration of 20 nM and oligonucleotide strands at 200 nM each. The folding buffer included 5 mM tris, 1 mM EDTA, 5 mM NaCl (pH 8), and 20 mM MgCl2. The reaction mixtures were subjected to a thermal annealing ramp using Tetrad (MJ Research, now Bio-Rad) thermal cycling devices. Oligonucleotides were purchased from Eurofins MWG. See Table 1 for folding ramps used to assemble the objects described in this study.

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